Journal: British Journal of Pharmacology

Article Title: Galantamine is not a positive allosteric modulator of human α4β2 or α7 nicotinic acetylcholine receptors

doi: 10.1111/bph.14329

Figure Lengend Snippet: Galantamine testing at the α7 nACh receptor expressed in HEK293 cells. Patch‐clamp electrophysiology recordings were performed on α7 nACh receptor stably expressed in HEK293 as described in Methods. (A, B) Representative traces of ACh‐evoked currents in the presence or absence of galantamine (A) or NS1738 (B) using the Dynaflow Resolve® system. Galantamine or NS1738 was initially applied for 40 s (60 s for the 0.01 μM concentration) and thereafter co‐applied with 80 μM ACh (~EC20) for 250 ms. The membrane potential was clamped at −70 mV. (C, D) Baseline‐subtracted peak current amplitudes were normalized to the prior AChcontrol application in the absence of galantamine/NS1738 as described in Methods. Normalized current amplitudes were plotted as a function of the galantamine/NS1738 concentrations and fitted to the Hill equation by non‐linear regression. Results from the fitting routine are indicated in the panels.

Article Snippet: HEK293 cell patch‐clamp recordings were performed using the Dynaflow Resolve® system (Cellectricon, Mölndal, Sweden) and an EPC10 USB amplifier (HEKA Electronik, Germany).

Techniques: Patch Clamp, Stable Transfection, Concentration Assay, Membrane